CellCelector Nanowell Arrays
Nanowell arrays for single cell isolation, screening and cloning
We have developed a wide range of innovative CellCelector™ nanowell arrays for single-cell screening and isolation as well as single cell cloning workflows to be used in various clinical, research and bioprocessing applications.
When used with the CellCelector™ single cell and clone picking system ALS nanowell consumables are easy to use and enable high-throughput and cost-effective alternatives to conventional limiting dilution, FACS and other single cell screening and cloning methods. Our nanowell arrays are available in various formats from 2 well chamber slides to 6 and 24 well plates. They are featuring from 100,000 to several millions of nanowells per plate/slide at nanowell volumes from several nanoliter down to a few picoliter.
CellCelector™ 4 nl nanowell plates for single cell cloning (S200)
CellCelector™ nanowell plates feature thousands of 200 µm square shape nanowells (S200) allowing cloning of hundreds or thousands of clones in parallel inside the nanowells. The volume of each nanowell is 4 nl which is sufficiently large for a clone of 50-100 cells (depending on cell size). The plates are available in standard SBS 6 and 24 well formats which allow running multiple experiments side-by-side. 6 well and 24 well plates feature respectively 135,000 and 100,000 nanowells per plate. An Ultra-Low Attachment (ULA) surface prevents cells from adhering to the surface and therefore allows 3D spheroid formation and a 100 % efficient automated single cell or clone transfer from the nanowells to regular cell culture plates for expansion or analysis.
Overview of a scanned well and different zoom levels down to a single nanowell (10x). The CellCelector allows automated scanning and detection of nanowells and cells located inside the wells.
CellCelector™ S200 ULA nanowell plates are ideal for the following workflows:
- Single cell cloning after gene editing (e.g. stem cells like iPSC etc.)
- Isolation of sets of defined cell numbers (e.g. 1, 5, 20, 50, 100) for reference samples
- Spheroid formation, imaging and screening
The 24 well plate offers a cost-efficient solution for processing multiple samples in parallel. Each well allows clonal growth of more than 500 single cells into clones.
Specifications
Product | Plate S200-100 72K, 24 well, ULA |
Catalog number | CC0079 |
Plate dimensions (mm) | 128 x 86 x 22 |
Plate material | PS |
Number of wells |  24 |
Nanowell volume | 4nl |
Nanowell shape |  Square |
Nanowell size | 200 x 200 µm |
Nanowell depth | 100 µm |
Nanowells per well | 3,000 |
Nanowells per plate | 72,000 |
Nanowell surface treatment | ULA |
Recommended glass capillary tip diameter | 80 µm |
S200 nanowell plates are available also with a plasma treated nanowell surface suited for adherent cell culturing (product numbers CC0075 for 24 well plates and CC0073 for 6 well plates, see ordering information below).
CellCelector™ sub-nanoliter nanowell arrays for single cell screening and rare single cell isolation (H100, U40, U25)
Arrays of sub-nanoliter nanowells provide a next generation system for screening and isolation of single cells. We have developed a range of nanowell arrays with nanowell sizes currently ranging from 100 µm (0.9 nl) to 25 µm (12 pl volume). 100 µm nanowells feature a hexagonal shape providing high packing efficiency of nanowells and easy picking. They can be also used for single cell cloning up to approximately 16 cells per clone. Smaller 40 µm nanowells allow for co-location of different cell types in the same wells for functional studies or receptor/reporter cell assays. Finally 25 µm and smaller nanowells provide the isolation and analysis of rare single cells like circulating tumor cells (CTCs)/fetal cells or the screening of antibody/protein producing cells (e.g. B-Cells) through secretion assays. Effectively separating single cells in small wells with a defined spacing allows extremely high seeding densities of tens of thousands of cells without compromising on the ability to screen, analyze or to pick them individually. All small nanowells have an optimized and patent pending UFO design providing 100 % single cell recovery efficiency.
H100 hexagonal nanowell array with grown hybridoma clones
(0.9 nl/nanowell)
U25 UFO nanowell array loaded with circulating tumor cells
(12 pl/nanowell)
Sub-nanoliter nanowell arrays are offered in two standard formats: 6 well plate and 2 well chamber slide.
Nanowell 6 well plate
Nanowell 2 well slide
Specifications
Product | Plate H100-100 330K, 24 well | Plate H100-100 360K, 6 well | Plate U40-40 850K, 24 well | Plate U40-40 1M, 6 well | Slide U40-40 200K, 2 well | SlideU25-25 400K, 2 well |
Catalog number | CC0084 | CC0080 | CC0096 | CC0097 | CC0083 | CC0081 |
Dimensions (mm) | 128 x 86 x 22 | 128 x 86 x 22 | 128 x 86 x 22 | 128 x 86 x 22 | 25.5 x 75.5 x 10.8 | 25.5 x 75.5 x 10.8 |
Number of wells | 24 |  6 | 24 | 6 |  2 | 2 |
Nanowell volume | 0.9 nl | 0.9 nl | 50 pl | 50 pl | 50 pl | 12 pl |
Nanowell shape |  Hex | Hex |  UFO (winged circle) | UFO (winged circle) |  UFO (winged circle) | UFO (winged circle) |
Nanowell size | 100 µm | 100 µm | 40 µm | 40 µm | 40 µm | 25 µm |
Nanowell depth | 100 µm | 100 µm | 40 µm | 40 µm | 40 µm | 25 µm |
Nanowells per well | 14,000 | 60,000 | 36,000 | 160,000 | 50,000 | 200,000 |
Nanowells per plate / slide | 330,000 | 360,000 | 850,000 | 1,000,000 | 200,000 | 400,000 |
Nanowell surface | Non-treated | Non-treated | Non-treated | Non-treated | Non-treated | Non-treated |
Recommended glass capillary tip diameter | 50 µm | 50 µm | 30 µm | 30 µm | 30 µm | 20 µm |
CellCelector sub-nanoliter arrays are ideal for the following workflows:
- B-cell screening through secretion assays in the nanowells for Antibody Discovery
- Hybridoma single cell cloning and screening
- Functional assays studying cell-to-cell interaction (e.g. T-Cells etc.)
- Rare cell isolation (CTCs, fetal cells, etc.)
- Cluster isolation
SIEVEWELL™ nanowell arrays for rare single cell isolation workflows
The SIEVEWELL™ chip consists of a thin membrane with nanowells of 20 µm in size and 25 µm in height each. In total there are 370,000 wells on the chip on an area of 17 x 17 mm. The outer dimensions of the chip correspond to the standard microscope slide format. Within each nanowell there are 2 pores with a diameter of 2 µm. The hexagonal shape of the wells is ideal for automated cell detection and cell counting. The membrane is characterized by a very high transparency as well as very low autofluorescence and is therefore ideally suited for microscopic and optical measurements.
Sievewell™ chip
Microscopic view of nanowell structure with 2 pores in each well
Specification
Product | SIEVEWELL Slide 370K | SIEVEWELL Slide 90K |
Catalog number | CC0085 | CC0098 |
Dimensions (mm) | 25 x 75 x 12 | 25 x 75 x 12 |
Working volume (ml) | 0.5 - 2 | 0.5 - 2 |
Number of wells per slide | 1 | 1 |
Nanowell volume | 8.6 pl | 108 pl |
Nanowell shape | Hex | Hex |
Nanowell size | 20 µm | 50 µm |
Nanowell depth | 25 µm | 50 µm |
Nanowells per slide | 370,000 | 90,000 |
Nanowell surface | Non-treated | Non-treated |
Recommended glass capillary tip diameter | 20 µm | 20 µm |
Due to its unique sieve structure SIEVEWELL™ arrays are a simple and perfect solution for
- On-chip staining of rare single cells, like circulating tumor cells, fetal cells etc. without any cell loss
- Single cell capture from cell suspensions: just pipetting. No pumps are needed.
Ordering information
All nanowell products are individually packed and sold by quantities of 5. Please contact us for other combinations of nanowell designs and plate or slide formats not listed below.
Catalog No. | Format | Description | Qty/pack |
Nanowell design S200, 4 nl/nanowell | |||
CC0079 | | CellCelector Nanowell plate S200-100 100K, 24 well, ULA | 5 |
CC0075 | | CellCelector Nanowell plate S200-100 100K, 24 well, Plasma treated | 5 |
Nanowell design H100, 0.8 nl/nanowell | |||
CC0084 | | CellCelector Nanowell plate H100-100 330K, 24 well, Non-treated | 5 |
CC0080 | | CellCelector Nanowell plate H100-100 360K, 6 well, Non-treated | 5 |
Nanowell design U40, 50 pl/nanowell | |||
CC0096 | | CellCelector Nanowell plate U40-40 850K, 24 well, Non-treated | 5 |
CC0097 | | CellCelector Nanowell plate U40-40 1M, 6 well, Non-treated | 5 |
CC0083 | | CellCelector Nanowell slide U40-40 200K, 2 well, Non-treated | 5 |
Nanowell design U25, 12 pl/nanowell | |||
CC0081 | | CellCelector Nanowell slide U25-25 400K, 2 well, Non-treated | 5 |
Nanowell design U15, 2.7 pl/nanowell | |||
Contact us | |||
SIEVEWELL™ Slide | |||
CC0085 |  | SIEVEWELL slide, 370K, 1 well, Non-treated | 5 |
CC0098 | | SIEVEWELL slide, 90K, 1 well, Non-treated | 5 |
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