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CellCelector Nanowell Arrays

Nanowell arrays for single cell isolation, screening and cloning

We have developed a wide range of innovative CellCelector™ nanowell arrays for single-cell screening and isolation as well as single cell cloning workflows to be used in various clinical, research and bioprocessing applications.

When used with the CellCelector single cell and clone picking system ALS nanowell consumables are easy to use and enable high-throughput and cost-effective alternatives to conventional limiting dilution, FACS and other single cell screening and cloning methods. Our nanowell arrays are available in various formats from 2 well chamber slides to 6 and 24 well plates. They are featuring from 100,000 to several millions of nanowells per plate/slide at nanowell volumes from several nanoliter down to a few picoliter.

CellCelector™ 4 nl nanowell plates for single cell cloning (S200)

Nanowell array plates 6 and 24 well
CellCelector™ nanowell plates feature thousands of 200 µm square shape nanowells (S200) allowing cloning of hundreds or thousands of clones in parallel inside the nanowells. The volume of each nanowell is 4 nl which is sufficiently large for a clone of 50-100 cells (depending on cell size). The plates are available in standard SBS 6 and 24 well formats which allow running multiple experiments side-by-side. 6 well and 24 well plates feature respectively 135,000 and 100,000 nanowells per plate. An Ultra-Low Attachment (ULA) surface prevents cells from adhering to the surface and therefore allows 3D spheroid formation and a 100 % efficient automated single cell or clone transfer from the nanowells to regular cell culture plates for expansion or analysis.
200 µm nanowell - different zoom levels
Overview of a scanned well and different zoom levels down to a single nanowell (10x). The CellCelector allows automated scanning and detection of nanowells and cells located inside the wells.
CellCelector™ S200 ULA nanowell plates are ideal for the following workflows:

  • Single cell cloning after gene editing (e.g. stem cells like iPSC etc.)
  • Isolation of sets of defined cell numbers (e.g. 1, 5, 20, 50, 100) for reference samples
  • Spheroid formation, imaging and screening
   
The 24 well plate offers a cost-efficient solution for processing multiple samples in parallel. Each well allows clonal growth of more than 500 single cells into clones.
Specifications
Product
Plate S200-100 100K, 24 well,
ULA
Plate S200-100 130K, 6 well,
ULA
Catalog number
CC0079
CC0069
Plate dimensions (mm)
128 x 86 x 22
128 x 86 x 22
Plate material
PS
PS
Number of wells

24

6
Nanowell volume
4nl
4nl
Nanowell shape

Square
Square
Nanowell size
200 x 200 µm
200 x 200 µm
Nanowell depth
100 µm
100 µm
Nanowells per well
4,300
22,600
Nanowells per plate
100,000
135,000
Nanowell surface treatment
ULA
ULA
Recommended glass capillary tip diameter
80 µm
80 µm
S200 nanowell plates are available also with a plasma treated nanowell surface suited for adherent cell culturing (product numbers CC0075 for 24 well plates and CC0073 for 6 well plates, see ordering information below).

CellCelector™ sub-nanoliter nanowell arrays for single cell screening and rare single cell isolation (H100, U40, U25)

Arrays of sub-nanoliter nanowells provide a next generation system for screening and isolation of single cells. We have developed a range of nanowell arrays with nanowell sizes currently ranging from 100 µm (0.9 nl) to 25 µm (12 pl volume). 100 µm nanowells feature a hexagonal shape providing high packing efficiency of nanowells and easy picking. They can be also used for single cell cloning up to approximately 16 cells per clone. Smaller 40 µm nanowells allow for co-location of different cell types in the same wells for functional studies or receptor/reporter cell assays. Finally 25 µm and smaller nanowells provide the isolation and analysis of rare single cells like circulating tumor cells (CTCs)/fetal cells or the screening of antibody/protein producing cells (e.g. B-Cells) through secretion assays. Effectively separating single cells in small wells with a defined spacing allows extremely high seeding densities of tens of thousands of cells without compromising on the ability to screen, analyze or to pick them individually.  All small nanowells have an optimized and patent pending UFO design providing 100 % single cell recovery efficiency.
H100 hexagonal nanowell array
H100 hexagonal nanowell array with grown hybridoma clones
(0.9 nl/nanowell)
U25 UFO-shape nanowell array
U25 UFO nanowell array loaded with circulating tumor cells
(12 pl/nanowell)

Sub-nanoliter nanowell arrays are offered in two standard formats: 6 well plate and 2 well chamber slide.
6well nanowell plate
Nanowell 6 well plate
nanowell slide
Nanowell 2 well slide
Specifications
Product
Plate H100-100 360K,
6 well
Slide U40-40 200K,
2 well
SlideU25-25 400K,
2 well
Catalog number
CC0080
CC0083
CC0081
Dimensions (mm)
128 x 86 x 22
25.5 x 75.5 x 10.8
25.5 x 75.5 x 10.8
Number of wells
6

2

2
Nanowell volume
0.9 nl
50 pl
12 pl
Nanowell shape

Hex

UFO (winged circle)

UFO (winged circle)
Nanowell size
100 µm
40 µm
25 µm
Nanowell depth
100 µm
40 µm
25 µm
Nanowells per well
60,000
50,000
200,000
Nanowells per plate / slide
360,000
200,000
400,000
Nanowell surface
Non-treated
Non-treated
Non-treated
Recommended glass capillary tip diameter
50 µm
30 µm
20 µm
CellCelector sub-nanoliter arrays are ideal for the following workflows:

  • B-cell screening through secretion assays in the nanowells for Antibody Discovery
  • Hybridoma single cell cloning and screening
  • Functional assays studying cell-to-cell interaction (e.g. T-Cells etc.)
  • Cluster isolation

SIEVEWELL™ nanowell arrays for rare single cell isolation workflows

The SIEVEWELL chip consists of a thin membrane with nanowells of 20 µm in size and 25 µm in height each. In total there are 370,000 wells on the chip on an area of 17 x 17 mm. The outer dimensions of the chip correspond to the standard microscope slide format. Within each nanowell there are 2 pores with a diameter of 2 µm. The hexagonal shape of the wells is ideal for automated cell detection and cell counting. The membrane is characterized by a very high transparency as well as very low autofluorescence and is therefore ideally suited for microscopic and optical measurements.
Sievewell slide for rare single cell applications
Sievewell chip
Sievewell pores
Microscopic view of nanowell structure with 2 pores in each well
Specification
Product
SIEVEWELL Chip 370K
Catalog number
CC0085
Dimensions (mm)
25 x 75 x 12
Working volume (ml)
0.5 - 2
Number of wells per slide
1
Nanowell volume
8.6 pl
Nanowell shape

Hex
Nanowell size
20 µm
Nanowell depth
25 µm
Nanowells per slide
370,000
Nanowell surface
Non-treated
Recommended glass capillary tip diameter
20 µm
Due to its unique sieve structure SIEVEWELL arrays are a simple and perfect solution for

  • On-chip staining of rare single cells, like circulating tumor cells, fetal cells etc. without any cell loss
  • Single cell capture from cell suspensions: just pipetting. No pumps are needed.

Ordering information

All nanowell products are individually packed and sold by quantities of 5. Please contact us for other combinations of nanowell designs and plate or slide formats not listed below.
Catalog No.
Format
Description
Qty/pack
Nanowell design S200, 4 nl/nanowell
CC0079

CellCelector Nanowell plate S200-100 100K, 24 well, ULA
5
CC0069
CellCelector Nanowell plate S200-100 135K, 6 well, ULA
5
CC0075

CellCelector Nanowell plate S200-100 100K, 24 well, Plasma treated
5
CC0073

CellCelector Nanowell plate S200-100 135K, 6 well, Plasma treated
5
Nanowell design H100, 0.8 nl/nanowell
CC0080

CellCelector Nanowell plate H100-100 360K, 6 well, Non-treated
5
Nanowell design U40, 50 pl/nanowell
CC0083

CellCelector Nanowell slide U40-40 200K, 2 well, Non-treated
5
Nanowell design U25, 12 pl/nanowell
CC0081

CellCelector Nanowell slide U25-25 400K, 2 well, Non-treated
5
Nanowell design U15, 2.7 pl/nanowell
Contact us
SIEVEWELL™ Chip
CC0085
SIEVEWELL chip, 370K, 1 well, Non-treated
5
Do you want to order or need more information?
                 
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